Hi Don, we isolated full-length mRNA from sorted cells after fixation and / or staining for intracellular antigens like PCNA. It is published in Journal of Biochemical and Biophysical Methods 40 (1999) 69-80 Isolation of full-size mRNA from cells sorted by flow cytometry by Diez et al. Hope this helps Andreas My address old: until 31.12.00 PD Dr. Andreas Simm Institut fuer Klinische Biochemie und Pathobiochemie Versbacher Str. 5 D-97078 Wuerzburg Germany Tel.: +49 931-201-3144 FAX.: +49 931-201-3153 E-mail: simm@klin-biochem.uni-wuerzburg.de New since 01.09.00 PD Dr. Andreas Simm Universitaet Halle Klinik fuer Herz- und Thoraxchirurgie Ernst-Grube-Str. 40 06120 Halle Tel.: +49-(0)345-557-2647 Fax.: +49-(0)345-557-2782 Email: andreas.simm@medizin.uni-halle.de
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