Dear Maryalice, This CD103 positive interesting case may be a case of Hairy Cell Leukaemia - Variant. We have not seen CD103 expression on PLL, but your results are consistent with a HCL-V, the nucleous in HCL-V may look like a B-PLL and the villi may have been "lost" because the smear is not fresh. BM trephine histology will give more information and help in the diagnosis. Ricardo. On Fri, 2 Feb 2001 11:00:27 -0500 Maryalice Stetler-Stevenson <stetler@box-s.nih.gov> wrote: > We have a very interesting case. An 80 year old gentleman > with a WBC= 47 K/uL and a working diagnosis of CLL sought another > opinion. We found his tumor cells to be negative for CD5 and CD23. > They had Bright CD19, CD20, CD22 and surface kappa. They were > positive for CD103, FMC7 & and CD11c, although the CD11c was not the > bright staining characteristic of hairy cell and CD25 was negative. > Also- cells were negative for CD38 and CD10 but + for CD79 and CD24. > The specimen was a submitted heparinized tube- no fresh smear. We > have found that cells sitting in heparin for awhile have poor nuclear > features after smearing and staining. However, the cells have copius > cytoplasm. Borders may be curled, indistinct, possibly hairy but > maybe not. We feel that this is most consistent with atypical hairy > cell. The diagnosis of CLL was never confirmed by immuno studies- > just a low grade lymphoid leukemia- and I feel the diagnosis is > wrong. The clinician asked if this could be PLL. Has any one ever > seen really good staining with CD103 in PLL? > > > Maryalice > > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > > "Learn the rules so you know how to break them properly." > The Dalai Lama ========================================================== Mr. Ricardo Morilla Academic Department of Haematology & Cytogenetics The Royal Marsden Hospital 203 Fulham Road, London SW3 6JJ, UK Tel +44 (0) 20 7808 2882; Fax +44 (0) 20 7351 6420 ==========================================================
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