I'm not surprised by this. You should do a titration to see what's optimal for you. I found that 100 nM worked great for me. "Henry H. Wortis" wrote: > Would someone with experience using CFSE, particularly with murine B cells, confirm > the correct dose? The one published > study with B cells that I found states that a dose of 10 nM/ml was used. This seems > to be about 1000 fold less than the > usual dose. > > Henry H. Wortis, M.D., Professor > Department of Pathology, > Director, Graduate Program in Immunology > Tufts University School of Medicine > 136 Harrison Ave. > Boston MA 02111 > Phone 617-636-6718 > FAX 617-636-2990 -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu
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