Dear Franck, You are exactly right: the condensation of the sperm chromatin accounts for the lower stainability. In fact people are using FCM/DNA staining to follow in vitro induced decondensation. This is important since failure of sperm decondensation results in failure of fertilization! See for instance Human Reproduction 10:1280-1286, 1995 and 11:837-843, 1996 and many other articles. I hope this helps, Best Regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Head Flow Cytometry Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium phone 32 3 821 3900, fax 32 3 825 1148 > ---------- > Van: Franck Morel[SMTP:F.Morel@univ-poitiers.fr] > Verzonden: donderdag 21 december 2000 15:38 > Aan: Cytometry Mailing List > Onderwerp: Sperm cells DNA content > > > Dear all, > An investigator in the lab try to stain sperm cells in order to > differentiate spermatozoid, aploid germinal cells and diploid non germinal > cells. First we tried to stain simultaneously spermatozoid and lymphocytes > using PI (Vindelov staining technic) and found a 4.6 fluorescence > intensity > ratio between the two populations. Can DNA structure (compaction, > protamines vs histones) explain this observation? Any comments will be > welcome. > Thanks in advance. > Franck. > Franck Morel > Laboratoire Cytokines > ESA CNRS6031 > IBMIG, UFR SFA > 40 Av du recteur Pineau > 86022 Poitiers Cedex > Tel: (33) 05.49.45.40.54 > Fax: (33) 05.49.45.35.03 > E-mail: F.Morel@univ-poitiers.fr >
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