Hello everyone... We have a group at NIH doing side population analysis using Hoechst 33342 by the standard technique. My (probably obvious) question is...is there something special about the Hoechst dye (mdr type specificity, nuclear localization, etc) that makes it the only mdr susbtrate effective for stem cell identification? Is it possible to use other fluorescent mdr substrates (i.e. rhodamine 123, daunomycin, etc.) to identify these cells? Thanks in advance! Bill Telford DCS-NCI-NIH
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