Hi Joseph, I guess you want to see some subsets of memory/activated and
naive T cells (by default), is that right?
I suggest the following tubes if you can have four colors
a) One tube with CD3/CD4/CD45RO/CD62L
b) One tube with CD3/CD8/CD45RO/CD62L
Then you will only see T cell subsets
If you can have only three colors
a) One tube with CD8/CD45RO/CD62L
b) One tube with CD4/CD45RO/CD62L
In this combination, there is the possibility that you can see other cells
that can carry CD45RO.
I suggest to do the CD8 because the CD8 part of the T cells have not been
so deeply evaluated.
Regards Rafael
>Hello Flowers,
>
>Which whole blood labeling scenario gives cleaner results?
>
>a) One tube with CD4/CD45RO/CD62L
>
> or
>
>b) Two tubes tube 1 - CD3/CD4/CD45RO
> tube 2 - CD3/CD4/CD62L
>
>Are there disadvantages/advantages to one or the other?
>
>
>Thank You,
>
>Joseph Idso
>Biologist
>UDSA-ARS-GFHNRC
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:14 EST