Hi Maciej, I thank you for your message. Most of the CD8 and CD4 cells are
from T cell lineage. However, there is a significant number of non T cells
that still express CD4 or CD8, for example a subsets of Dendritic cells
from mieloid origin express CD4, Another subset of lymphoid dendritic cells
also express CD8. You can argue that those subsets are in minute proportion
in regard with the bulk of the T cells. However, when you go into the
gating of the subsets for CD4 or CD8, what it could be about 1% turn out
being higher. There is a recent reference form the DC meeting in Australia
published in immunology today. "Dendritic cells: multilineal and
multifunctional, by Grabbe S, Kämpgen E. and Schuler G. Immunology today,
september 2000, No 9 431.
Also, there is the group of killer cells that carry CD8 but they are not T
cells.
I hope this comments help. Regards Rafael
>you could eliminate CD3 from that and stick to 3 color if you have a
>good separation between NK CD 8 and T cell CD8 as well as monocyte
>CD4 and T cell CD4, both based on brightness. Am I wrong in assuming
>that all bright CD8/CD4 will also be CD3+ ?
>
>Maciej
>--- Rafael Nunez <rafaeln@vetvir.unizh.ch> wrote:
>>
>> Hi Joseph, I guess you want to see some subsets of memory/activated
>> and
>> naive T cells (by default), is that right?
>> I suggest the following tubes if you can have four colors
>> a) One tube with CD3/CD4/CD45RO/CD62L
>> b) One tube with CD3/CD8/CD45RO/CD62L
>> Then you will only see T cell subsets
>>
>> If you can have only three colors
>> a) One tube with CD8/CD45RO/CD62L
>> b) One tube with CD4/CD45RO/CD62L
>> In this combination, there is the possibility that you can see
>> other cells
>> that can carry CD45RO.
>>
>> I suggest to do the CD8 because the CD8 part of the T cells have
>> not been
>> so deeply evaluated.
>> Regards Rafael
>>
>> >Hello Flowers,
>> >
>> >Which whole blood labeling scenario gives cleaner results?
>> >
>> >a) One tube with CD4/CD45RO/CD62L
>> >
>> > or
>> >
>> >b) Two tubes tube 1 - CD3/CD4/CD45RO
>> > tube 2 - CD3/CD4/CD62L
>> >
>> >Are there disadvantages/advantages to one or the other?
>> >
>> >
>> >Thank You,
>> >
>> >Joseph Idso
>> >Biologist
>> >UDSA-ARS-GFHNRC
>>
>>
>> \|/
>> (o o)
>>
>________________________________oOo__(_)__oOo_________________________________
>> ___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
>> / o \/| | University Inst.for Virology
>> http://www.vetvir.unizh.ch/
>> / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
>> /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1
>> 6358911
>>
>______________________________________________________________________________
>>
>>
>>
>>
>>
>>
>>
>
>
>=====
>`---------------------------------------------`
>| Maciej S. Simm | 525 E 68th Street |
>| Research Technician | Room N-805 |
>| Cornell Medical Center | Tel. 212.746.3428 |
>`---------------------------------------------`
>|www.cd4cd8.com | 917-734-6280 | AIM - XsimmX |
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\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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