Dear flowers: I am working on ApoE knockout mice that develop atherosclerosis and would like compare the realtive percentage of macrophages in mouse splenocytes and peripheral blood samples and also measure cytokine levels, specifically TNF alpha and IL-6, in activated macrophages. What are the good Macrophage markers available for FACS. We tried CD107b as a Mac tag but couldn't detect anything, possibly due to sheer inexperience. For those of you folks who are macrophage and cytokine savvy...please help! We would also like to look at ox-LDL scavenger receptors on these macs. Could my macs be sticking to the plastic tubes I use for flow. Are they any particular staining techniques required? I used the Pharmingen staining protocol for whole blood. I would really appreciate some advice on how to proceed into this sticky realm. We use a dual laser FACS Calibur. Literature references, protocols and suggestions are welcome. Thanks Yasmin Marikar
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