Thanks for all the mails , it was great help Now I complete the information, after check all my protocols and after wash the sample and incubate with PBS-SFB 1% , and some things change: Th patien is a man, 21 years, that begin 2-3 weeks ago with a like aplasia evolution, but last week the WBC increase and appears abnormal cell. The sample was periphereal blood, WBC, 23.000, with a very little atypical cells. The population of this litlle cells (low FSC, low SSC) and low expresion of CD45 (Tipical region for B leukemias) is about 52.79% and was CD19(-), CD20(-), CD10(-), CD34(-),CD13(-), CD14(-), CD33(-), HLA-DR(-), CD16(-), CD56(-), CD3(-), CD4(+), CD8(+), CD2(+), CD7(+), CD5 dim, MPO (-), TdT (+) and CD3cit.(+). Finnaly I think that the diagnosis is clear T-LLA Thanks Again JUAN LUIS CASTILLO N. CITOMETRIA DE FLUJO HOSPITAL DEL TRABAJADOR CARDENIO AVELLO 36 CONCEPCION CHILE: PHONE: 56-41-402138 56-41-329454 FAX: 56-41-402117 E- MAIL: axelyoyi@entelchile.net http://oncoinmun.bizland.com
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