Hi everybody,
My question is probably related to the isotype control/autoflourescence
question. I am comparing several different cell types, for expression of
of the same marker X. The catch is that the intensity of staining of the
same isotype control is different in each cell type.
The different cell types clearly show different intensities of staining
with marker X, and this would correlate with what I see functionally.
However, as I said before the isotype controls also increase somewhat,
though not as strongly in intensity.
What is the best way to compute numbers for this case?
1. Show median intensity for the total populations, without subtracting
the isotype?
2. Use K-S stats to compare marker X and isotype control on the same cell
type? Would someone please enlighten me on this one? Which figure is
relevant? D/S(n) or the channel figures?
3. Cell quest has an option whereby I can get stats using the marker X as
a numerator and the isotype control as a denominator? Is this an option?
Forgive my ignorance, and any suggestions/explanations would be much
appreciated.
Meenakshi Roy
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:10 EST