> ---------- > From: andrew.head@covance.com > Sent: Saturday, 21 October 2000 4:43 > To: Cytometry Mailing List > Subject: Intracellular ifn-gamma and IL-4/5 > > On the 21st October, Andy wrote: > Dear flowers > > I am trying to type Th1 and Th2 cells in Cynolmolgus macaque > lymphocytes using intracellular IFN-gamma, IL-4 and IL-5 > Does anyone have any experience of this ? > Using PMA activation(5 hours plus monensin) I have had some success > with IFN-gamma, but no luck with IL-4 or 5.Am I supposed to use a > different activator for these two monoclonals to work? or incubate > for > a different length of time? > Any suggestions would be appreciated. > > Thanks > Andy > -------------------------------------------------------------------------- > --------------- > > Andy, > I havent worked with Cynolmolgus macaque lymphocytes, but in the > case of human lymphocytes you will get a better signal following 24 h > incubation with PMA (25 ng/ml) + Ionomycin (1ug/ml) (with 1 ug/ml BA or > Mon Golgi block), especially for IL-4, IL-5. > Hope this helps. > > Regards, > > Greg Hodge PhD > email: hodgeg@mail.wch.sa.gov.au > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. >
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