Hi Carsten, Below are a couple of references that you might find useful. Your approach really depends on the type of instrumentation you have available. Good luck, ~jmb~ Stull RA. Hyun WC. Pallavicini MG. Simultaneous flow cytometric analyses of enhanced green and yellow fluorescent proteins and cell surface antigens in doubly transduced immature hematopoietic cell populations. Cytometry. 40(2):126-34, 2000 Jun 1 Beavis AJ. Kalejta RF. Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm. Cytometry. 37(1):68-73, 1999 Sep 1. on 8/31/00 4:56 AM, Lindemann, Carsten at CLindemann@eufets.com wrote: > > Clontech sells vectors for EYFP and EGFP. To analyse a mixture of EYFP and > EGFP positive cells > they recommend the use of a 525 nm dichroic short pass mirror together with > a 530/30 nm band pass and 510/20 bandpass filter. > Has anyone experience with the spectral separation of EYFP and EGFP? > > > Thanks for your reply > > Carsten Lindemann >
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