Clontech sells vectors for EYFP and EGFP. To analyse a mixture of EYFP and EGFP positive cells they recommend the use of a 525 nm dichroic short pass mirror together with a 530/30 nm band pass and 510/20 bandpass filter. Has anyone experience with the spectral separation of EYFP and EGFP? Thanks for your reply Carsten Lindemann
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