Hi David, If it's increased brightness you want for increased sensitivity detection of low levels of antigen then I would try the Alexa488 conjugated antibodies from Molecular probes. I compared FITC, PE and Alexa488 on a particular antigen that was expressed at low levels. The FITC didn't pick up anything and the Alexa fluorescence intensity was approximately 5 times brighter than PE. There's also an Alexa488 amplification kit which is supposed to be even brighter although I haven't used it yet. I hope that helps. Cheers, Laura. Laura Dash Glaxo Wellcome > -----Original Message----- > From: David_C_McFarland@sbphrd.com [SMTP:David_C_McFarland@sbphrd.com] > Sent: 18 August 2000 17:36 > To: Cytometry Mailing List > Subject: high sensitivity reagents > > > Good day to all. Sometime ago I received an add for antibody-fluorescent > conjugate that delivered on the order of 100 molecules of dye per binding > event. > )Alright, I don't know if was really 100, but it was a lot.) It was a big > phycobiloprotein construct as I recall. Does this ring a bell with > anyone? > I'm trying to detect very low expression of a surface antigen and thought > this > might be the way to go. The add must have gotten lost during my recent > move. I > hope someone can help because it's kinda hard to search for something if > you > don't know what it's called! > > David McFarland > SmithKline Beecham Pharmaceuticals >
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