> ---------- > From: Vivian Wu > Sent: Friday, 18 August 2000 13:22 > To: Cytometry Mailing List > Subject: Stimulating monocytes > > Vivian, > > I have noticed increased loss of CD14 and CD33 on monocytes with > increasing concentration of LPS used. > I have success stimulating i/c cytokines with 100 ng/ml LPS in a whole > blood 24 h assay and still have appreciable CD14 and CD33 staining. > > Regards, > Greg Hodge PhD > Flow Lab/ Haematology Department, > Women's and Children's Hospital > North Adelaide, > South Australia 5006 > ph: 08 82046522 > fax: 08 82046043 > email: hodgeg@mail.wch.sa.gov.au > -------------------------------------------------------------------------- > -------------------------- > > On 20/8/00 Vivian wrote: > Hi All, > > I have been stimulating monocytes with LPS and adding monensin into the > culture for the last 4 hours. The CD14 and CD33 antigens were down > regulated > after 24 hours of stimulation. I would like to be able to gate on > monocytic > antigens while doing cytokine analysis. Are there other monocytic antigens > which will not be down regulated after 24 hours of stimulation? I'd > appreciate any suggestions and thanks in advance. > > Vivian Wu > Immunology Lab. > Vancouver General Hospital > B.C. > email: ffwu@interchange.ubc.ca >
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