Hello Alberto, look at the following Web side: http://www.molbio.princeton.edu/facility/flowcyt/cytometers.html the people at the FLOW CYTOMETRY CORE FACILITY (Andrew Beavis, Manager Tel: (609) 258 1695 Fax: (609) 258 5323 Abeavis@molbio.princeton.edu) use a 4 Color FACScan with FITC,PE, PECy5 (Cychrome) or PE-APC and PE-Cy7 for FL4. The Four-colour FACScan is a custom product from Becton-Dickinson Immunocytometry Systems. I asked my local Becton-Dickinson sales person here in Germany for this option to upgrade our FACScan, but he told me that this option has no CE Sign and so it will not available in europe. Sometimes it seems to me that Becton-Dickinson have a lot of options for the Cytometers, but they want to sell it only to selected peolpe. I think a modification for a Calibure is easier and you need some new dicroic mirrors and filters. The best source for filters are the two companies in Brattleboro, Vermont Omega Optical, Inc. ( http://www.omegafilters.com/products/fluorescence/fforf.html ) and Chroma ( http://www.chroma.com/ ) This companies make custom filters for a good price. I hope this will help you, please ask the people at princeton foe details. With best wishes Christoph Goettlinger Christoph Goettlinger Institute for Genetics University Cologne Weyertal 121 50931 Koeln Germany
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