Jenny, We have seen two such patients in our laboratory. Both were HIV+, but the fluorescence had nothing to do with their antiretroviral therapy. Both exhibited extremely bright autofluorescence in the FITC channel only (unstained cells). We did not try washing first. A phone call to the referring physicians revealed that both had recently (within 48 hours) undergone fluorescein angiography by an ophthalmologist to rule out CMV retinitis. I did a literature search at the time, and, to my knowledge, that phenomenon had not been published. all best, David David Sadler, MD Warde Medical Laboratory Ann Arbor MI ++++++++++++++++++++++++++++++++++ >>Hello to all, I have just tested a patient on antiretroviral therapy whose cells fluoresced to the extent that compensation was impossible. This has prompted 2 questions, 1. Does anyone know of a list of drugs (prescribed or "recreational") which cause this phenomenon? 2. How do you deal with these patients? Do you lyse and wash before staining? If so, how would this effect your results? Thanks Jenny Jenny Bryant Flow Cytometry Australian Red Cross Blood Service - NSW/ACT 153 Clarence St Sydney, NSW 2000, AUSTRALIA .._|\ Ph: 61 2 9229 4341 / \ FAX: 61 2 9229 4521 \_.-._/<<<<<< E-mail: jbryant@arcbs.redcross.org.au v
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