ANSWER TO I have an ambitious postdoc who would like to analyze the protein content of exosomes by FACS. He tells me that exosomes range in size from 50 - 100 nM. Has anyone ever done this? or know if it is possible to do FACS analysis on "cells" this small? We have access to a FacScan, a FacScalibur, a FacsVantage and a MoFlo - any thoughts on which machine might be best for this analysis? I am afraid the vesicles will be at the limit of detection of most of the flow cytometers in size. But never mind, just try.... The light scattering under the wavenlength of the laser is special and you might be able to find some indications both in fSC and SSC (90°C). For te proteoins, as soon as you are able to stain them, their should not be any problems. For your knowledge, concening the exocytotic vesicles originating from dictyostelium discoideum, they could be recorded in the place where we usually consider the debris an characterized for their fluorescence. The student you have is not specially ambicious, he just ask questions which could have answers! Good luck and cotact me if needed Patrice \ | / (o o) \ | / _______________oOo_____oOo_____oOo_ (o o)__oOo________________ Dr. Petit Patrice X. Institut Cochin de Génétique Moléculaire INSERM U129 - CHU Cochin Port-Royal 24, rue du Faubourg Saint-Jacques F-75014 Paris, France. Tel: 33 01 44 41 24 11 Fax: 33 01 44 41 24 21 E-mail: pxpetit@icgm.cochin.inserm.fr
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