What is a Vacutainer CPT? How does it substitute for Ficoll separation? The protocol
that was referred to mentioned K3 EDTA Vacutainer blood collection tubes. Are these the
Vacutainer CPT? When I was in the BD website, I found about a zillion matches for
Vacutainer
and when I narrowed it with CPT there were far fewer but these had a relevance of 18% at
most. When I looked in my BD catalog, there was no mention of Vacutainers for sale. Very
puzzling.
Kevin G. Waddick, Ph.D.
Parker Hughes Cancer Center
Bernhard Peball wrote:
> Dear Sathi,
>
> I use the lysis protocol mentioned on the "BD Monoclonal Antibodies Source Book" CD.
> It's very simple and the viability of the cells is good. I avoid Ficoll, if
possible. Too
> tedious.
> A Vacutainer CPT does the same and is a lot easier in handling.
> If you don't have the BD CD, you can find the protocol at
> http://www.bdfacs.com/source_book/html/23_1372n.shtml
> Anyway, if someone has a better one, I'm always open to improvements.
>
> BTW: is there any alternative to BD CPT- Vacutainers? The product is just fine,
> but our local BD representative could need a little competition :-)
>
> Yours, brn
>
> On Tue, 16 May 2000 12:19:54 -0400, Sathi wrote:
> >
> >Hi there:
> >
> >We have been doing indirect immunofluorescent surface staining for
> >phenotyping (against surface markers) on PBMC isolated from blood. We
> >isolate PBMC from heparin added blood using Ficoll-hypaque (Pharmacia)
> >gradient centrifugation method. Now our lab is considering using whole
> >blood staining method since lot of others suggested that we might be losing
> >some minor cell sub-populations during Ficoll-centrifugation and
> >recommended whole blood staining. Since I have not done whole blood
> >staining method so far, could people who are already doing whole blood
> >staining method tell me where I can get the protocol (any useful
> >references), any technical tips in adopting the method, any tips on making
> >the lysis reagent (buffer). I would appreciate very much any help on this
> >methodology.
> >
> >
> >Thanks in advance
> >
> >Sathi
> >-------------------------------------
> >e-mail: sathy@oitunix.oit.umass.edu
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