A probably stupid question for the cell-cycle gurus: When doing cell cycle analysis (PI stained cells) here and then I have a sample which for no apparent reason shifts in staining intensity when compared to the others. How do you adjust for this if you do not use such a program as Modfit to calculate the percentages of the different stages? Do you correct the PMT voltage during aquisition in order to get the G1 peaks at the same positon or do you change the position of the markers at analysis (Or do you throw away the data of those samples)? If you change the markers, do you do do this just by eye or do you use a certain algorithm to do this? Is somebody including references as RBC nuclei? Any answers appreciated Greetings Claudio =================================================== Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone Office: 031 / 632 99 68 University of Bern E-Mail: vallan@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31 Insel hosptial area only: 3010 Bern Beeper: 181 67 59 ===================================================
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