In peripheral blood, according to the classical scheme of T-cell
differentiation, mature CD4+ and CD8+ T-cells are mutually exclusive subsets.
However, mature T-cells with double-positive phenotype (CD4+/CD8+) can be found
in very small numbers (1-3%) in human, and rat peripheral blood, and in a higher
percentage, up to 15%, in rhesus monkeys (Macaca mulatta), cynomolgus monkeys (
Macaca fascicularis) and African green monkeys (Cercophitecus aethiops). In
normal adult pigs, a substantial proportion (10-60%) of peripheral lymphocytes
can simultaneously express CD4 and CD8. Coexpression of CD4 and CD8 can be
generated in peripheral blood T-cells by treatment with lectin which induces CD8
a biosynthesis and cell surface expression. The CD4+/CD8+ population derives
from CD4+/CD8- T-cells that become double positive upon activation.
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CD4+CD8+phenotype on activated T cells: possible role of antigen transfer.
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peripheral blood, splenic, and thymic lymphocytes in male and female rats.
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of porcine peripheral blood CD4/CD8 double-positive T cells. Immunology 87:500.
Currier, J.R., Stevenson, K.S., Kehn, P.J., Zheng, K., Hirsch, V.M. and
Robinson, M.A. (1999) Contributions of CD4+, CD8+, and CD4+CD8+ T cells to
skewing within the peripheral T cell receptor beta chain repertoire of healthy
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induce the expression of CD8 alpha chains on concanavalin A-activated rat CD4+ T
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Elevated levels of peripheral double-positive cells have previously been
reported in one apparently normal adult male, and this phenotype appear to be
increased in a number of clinical conditions, including myasthenia gravis,
multiple sclerosis, idiopathic thrombocytopenic purpura, Behcet?s syndrome,
HIV-infection, inflammatory bowel disease, Kawaski disease, lepramatous leprae,
and patients suffering some types of neoplasia.
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lymphocyte population co-expressing T4 (CD4) and T8 (CD8) antigens in the
peripheral blood of a normal adult male. Blood 75:2024.
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patients with Kawasaki disease. Clin.Exp.Immunol. 111:397.
Senju, M., K.C. Wu, Y.R. Mahida, and D.P. Jewell. 1991. Coexpression of
CD4 and CD8 on peripheral blood T cells and lamina propria T cells in
inflammatory bowel disease by two colour immunofluorescence and flow cytometric
analysis. Gut 32:918.
Mizuki, M., S. Tagawa, T. Machii, M. Shibano, E. Tatsumi, K. Tsubaki, H.
Tako, A. Yokohama, S. Satou, J. Nojima, T. Hirota, and T. Kitani. 1998.
Phenotypical heterogeneity of CD4+CD8+ double-positive chronic T lymphoid
leukemia. Leukemia 12:499.
Ottenhoff, T.H., D.G. Elferink, P.R. Klatser, and R.R. de Vries. 1986.
Cloned suppressor T cells from a lepromatous leprosy patient suppress
Mycobacterium leprae reactive helper T cells. Nature 322:462.
Weiss, L., A. Roux, S. Garcia, C. Demouchy, N. Haeffner-Cavaillon, M.D.
Kazatchkine, and M.L. Gougeon. 1998. Persistent expansion, in a human
immunodeficiency virus-infected person, of V beta-restricted CD4+CD8+ T
lymphocytes that express cytotoxicity-associated molecules and are committed to
produce interferon-gamma and tumor necrosis factor-alpha. J.Infect.Dis.
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Watanabe, N., S.C. De Rosa, A. Cmelak, R. Hoppe, L.A. Herzenberg, and M.
Roederer. 1997. Long-term depletion of naive T cells in patients treated for
Hodgkin's disease. Blood 90:3662.
Senju, M., K.C. Wu, Y.R. Mahida, and D.P. Jewell. 1991. Coexpression of
CD4 and CD8 on peripheral blood T cells and lamina propria T cells in
inflammatory bowel disease by two colour immunofluorescence and flow cytometric
analysis. Gut 32:918.
Jose Diaz Romero
Transplantation Deparment
Novartis Pharma AG
Basel
Switzerland
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