Re:sheath fluid for Calibur

From: Arnold Pizzey (a.pizzey@ucl.ac.uk)
Date: Thu Mar 30 2000 - 05:58:14 EST


>The question on sheath fluid is whether inexpensive water is equivalent to
>expensive saline. We use filtered water on a FASCalibur primarily for DNA
>analysis.  We have not seen any problems due to possible mixing of sample
with
>sheath. Using Salt has one potential problem. Salt must be cleaned from the
>system or it can cause some major  fluidic problems, namely blockage
(minor and
>major). Water is easier and cheaper to use.
>Bob
>
>Robert M. Zucker, Ph.D
>U.S. Environmental Protection Agency
>National Health and Environmental Effects Research Laboratory
>2525 NC Highway 54,  MD 72
>Research Triangle Park, North Carolina, 27709
>tel: 919-541-1585 fax 919-541-4017
>e-mail: zucker.robert@epa.gov
>

I have used water for my sheath fluid for the last 10 years result; less
blockages, no salt deposits, and no contamination on subsequent sterile sorts


Arnold

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	Arnold Richard Pizzey
	Department of Haematology
	Royal Free and University College London Medical School
	98 Chenies Mews
	London WC1E 6HX
	U.K

	voice:	+44 0207-209-6234
	Fax:	+44 0207-209-6222
	email:	a.pizzey@ucl.ac.uk
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