Dear Flowers, we are in the process to buy a FACSCalibur, and we would like to here your suggestion abut the kind of sheath fluid we must/can use. We aren't planning any sorting, maybe some DNA work, lot and lot of phenotyping and intracellular staining. >From the archives looks like that different people use different solutions, and for different reasons. I have been using sterile saline for FACScan and FACStar, and D'PBS for FACSort, but being new to the Calibur world, I would like to be sure that those alternatives to the BD sheath fluid are OK. Thanks a million in advance Mara Mara S.L. Rocchi Moredun Research Institute Penicuik EH26 0PZ Scotland, UK e-mail: roccm@mf.mri.sari.ac.uk
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