Hi, which conjugate for CD19 is used? PeCy5 conjugated MAbs often bind to monocytes giving unspecific double positive results. Best wishes Anna At 15:11 2000-03-07 -0800, you wrote: > Hello, I was wondering if anyone has seen a population positive for >both CD14 and CD19. I am staining peripheral blood lymphocytes and am >looking at the B cell population. I originally just used CD19 to get the B >cells. However recently I began using CD3, CD14 to exclude any T cells and >monocytes. I will use CD56 as well to exclude NK cells. However, I did see >some cells that were positive for both CD19 and CD14. Are these cells >monocytes or are they B cells. I do want to look at all B cells, my >question is how can I find out if these cells are truly B cells or if they >are monocytes? Thanks, Annuska Glas Anna Porwit Haematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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