Dear all, How does one define sort purity? Is there any consensus around how to present purity figures? To exemplify: If sorting a CD34+/CD38- "population" of 6% (based on the 34+ of the total viable cell population) and knowing how the machine works, a population rather corresponding to the ~25% lowest gets enriched. Within the original sorting gate approximately 60% of the events fall and the rest will fall above the sorting gate. These 40% are not something I would define as impurities since they are a part of the sorted cluster of CD34+/CD38- cells. How do I define sort purity? Is it 100% of the 25% lowest or 60% of the 6% lowest. It becomes pretty obvious that, if wanting to sort these very 6%, the sort gate should include perhaps the 1% lowest or something in that way. However, most users of sorted fractions are not aware of the limitations of the sorter but merely ask for the 6% lowest. This of course is a bit of a dilemma for the person operating the sorter since there are no simple algorithm to apply correcting for this phenomenon. How do others deal with this? Thanks for your input Carl-Magnus Högerkorp Stem Cell Laboratory University Hospital of Lund Sweden
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