Hi Mike,
There is a common snag in transfering to foreign file systems from Macs: in
an effort to preserve information on placement of icons, the notorious
resource fork, as well as the data all in one package, macbinary was
developed. This is often the default transfer format for mac ftp programs,
it allows transfer of mac files to other systems and then back to mac with
no loss of information. Unfortunately some of this information gets
inserted as a header before the actual data, and if opened on a PC etc, the
file appears corrupted.
The answer to this problem is to explicitly set the transfer type to Raw
Binary if transferring by ftp/http, or to use IBM format discs instead.
Binary transfer means that some macintosh OS information will be lost (eg
icon placement, file type and creator), although the data will stay intact,
disc transfers will keep that information in a directory and file hidden
from windows.
This may not be relevant, but I hope it helps,
Ray
At 6:22 am -0500 18/1/00, Michael Ormerod wrote:
>I had a request for help from Yanhui Yang in China in analysing DNA
>histograms.
>
>I suggested using WINMDI to create DNA histograms and then Cylchred to
>analyse them
>(see below). However, he was unable to read his data files using WINMDI.
>
>I have never had a problem reading BD files supplied to me by other people.
>I do not have direct access to a Cellquest system to explore this directly.
>Can anyone help?
>
>Thanks,
>
>Michael Ormerod
>
>-------------Forwarded Message-----------------
>
>From: Yanhui YANG, INTERNET:yhyang@jzw.cern.ac.cn
>To: Michael Ormerod, Michael_Ormerod
>
>Date: 17/01/00 23:09 PM
>
>RE: Re: help
>
>
>Dear Dr.Ormerod,
>
>Thank you very much for your kindness and the information provided. I've
>downloaded
>those free softwares and tried my data on them. But unfortunately, there was
>something wrong when the downloaded software WinMDI reading my data files.
>It was
>said that the files were not FCS files. Actually, the data were originally
>acquired
>in Japan by a FACSCalibur FCM with a software of CellQuest running on Mac,
>and then
>transfered into PC readable format for the reason that we do not have a
>Mac here. I
>wonder if it is this transfermation cause the error in reading the files?
>If so, how
>can I make the analyze feasible? I'll be very grateful if you would
>provide further
>help.
>
>Thanks again and sorry for the bother.
>
>Best wishes
>
>Sincerely,
>Yanhui
>
>In 00-1-17 17:00:00 you wrote:
>>If you have your data in a listed data file in FCS format (used by all
>>modern flow cytometry systems) you can produce histograms in FCS format
>>using WINMDI.
>>
>>You can then analyse these files using Cylchred.
>>
>>Both these programs are available free on the Internet.
>>
>>WINMDI is available from http://facs.scripps.edu.
>>
>>Cylchred is available from http://www.uwcm.ac.uk/uwcm/hg/hoy/software.html.
>>
>>
>>I hope that this is of help to you.
>>
>>Michael Ormerod
>>34 Wray Park Road
>>Reigate RH2 ODE
>>Telephone: +44 (0)1737 241726
>>PLEASE NOTE NEW FAX & MOBILE NUMBERS
>>FAX: +44 (0)1737 226736
>>Mobile telephone: 07802 293242
>>Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
>
>
Ray Hicks
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