Dear Flow-ers, I am using a polyclonal antibody (unpurified immune serum) for indirect staining which gives me a very bright signal on flow. The problem is that if I stain using serial dilutions of the serum (1:3, 1:10, etc.) the signal begins to decrease with the first dilution, gradually tapers off, and reaches the level of the negative control at about 1:10,000. Because the signal drops with the first dilution, I assume I have not achieved saturation with the antibody, even though I would expect there to be a high antibody concentration in the serum. Does anyone have any idea what's going on? Thanks in advance for any help. Art __________________________________ Arthur Roberts Dept. of Medicine Robert Wood Johnson Medical School email: robertar@umdnj.edu phone: 732-235-7790 Fax: 732-235-7792 -- End --
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