Is there anyone who has upgraded their FACStar Plus with the turbo sort option and is happy with it? My FACStar Plus was upgraded with the TSO last year. The most noticeable improvement was fewer clogs with the macro sort head. Hi-speed is a different story though. I am unimpressed with the TSO when it comes to running hi-speed. my first problem is with severe noise in the Side scatter PMT. I can minimize this noise with the SSC obscuration bar, but It is not a complete fix and I take a big hit in the signal itself. second, making decent side streams is a challenge. Even if I can make good side streams, often the distance to the media in the tube is great, and I suspect a substantial number of cells end up drying and dying on the side of the tube. Between these two problems, I seldom use the TSO. Unfortunately, I have been getting a lot of sorting requests that require sorting at Hi speeds. My request is two-fold. 1) any suggestions for the problems stated above. 2) I would like to get a general idea of how many users are satisfied with the performance of there Turbo Sort Option installed on the FACStar Plus. Brian Tilton Flow Cytometry Beth Israel Deaconess Medical Center Boston, MA 02215 tel# 617-667-4901
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:22 EST