Dear Are: You didn't mention what sorting procedure you are using (directly on slides or first into tubes followed by cytospin). In our lab/Flow CORE facility we routinely do FISH on cells sorted directly onto Silane-Prep slides (Sigma) on a cushion of 1% BSA in PBS, air dried, and fixed in 3:1 methanol/acetic acid, with excellent results. This method has been published: Engel et al. (1997): Br.J.Haem. 99, 64-75. Hope this helps. Doug Weidner, Ph.D. UT MD Anderson Cancer Center Houston, Texas, USA
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