Hello all flowpeople! I have experienced a problem and wonder if anybody can help me. I have sorted MNC cells by flow on a FACSVantage and then performed FISH. My problem is that the FISH result is very poor on the cells after been through the FACSVAntage. I do not know what cause the problem. FISH on the same cells before the flow procedure (using Mab's on them and there after perform flowsorting) is perfect Have anyone experienced the same kind of problem and hopefully have a solution on the problem?? Best regard Are P. Normann Institute of immunology The national hospital, Oslo Norway a.p.normann@labmed.uio.no
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