Jim, I published an activation paper concerning asymptomatic HIV-infected individuals in 1997, Cytometry ( Comm.) 30:1-9. We found the earliest marker of activation, regardless of the cell type was the CD69 marker. In fact, after 1-2 hours of stimulation this marker is measurable from backgroud. To date, we incorporate this marker in our intercellular gamma interferon assays as well. However, I would also consider using CD45RA and CD62L. While stimulated with a polyclonal activator such as PHA the majority of cells will convert to a "memory" cell phenotype, CD45RA neg. CD62L+ usually in three days. In addition CD95 (FAS-Ag) is also a good stable marker yet it appears later than CD69. Currently we are activating cells over a 14 day period to study chemokine receptors, remarkably CxCR4 percentage and intensity corrleates well with proliferation I hope this info helps. Stephen P. Perfetto, MS.,MT. (ASCP) Walter Reed Army Institute of Research Department of Molecular Diagnostics and Pathogenesis 1600 East Gude Drive Rockville, MD. 20850 _______________________________________________________________________________ Subject: T cell activation markers From: "Houston; Jim " <Jim.Houston@stjude.org> at Internet_Gateway Date: 12/14/99 2:52 PM To All: Can someone point me in the correct direction with some possible articles as to what marker are needed to identify reactive to non reactive t-cells. These cells will be activated much like an MLC. What type of activation is expected? In the course of a 7 day activation what markers come and go? Any help is needed.. Jim Houston
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