We ran crypto when I was at the U. of IL. The only thing that inactivated
oocysts was the brown liquid lysol. Straight bleach wouldn't do it. We ran
live samples and then cleaned up afterward with the lysol, so I can't say what
they look like after lysol treatment. Crypto and Giardia related sicknesses
aren't fun, so be careful.
David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center
---------------------- Forwarded by David McFarland/VUMC/Vanderbilt on 12/13/99
02:50 PM ---------------------------
Neo Sim Yee <engp9476@nus.edu.sg> on 12/12/99 09:53:15 PM
(Embedded image moved to file: pic25436.pcx)From:(Embedded image moved to
file: pic27068.pcx)Neo Sim Yee <engp9476@nus.edu.sg> on 12/12/99 09:53 PM
To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
cc: (bcc: David McFarland/VUMC/Vanderbilt)
Subject: Giardia and cryptosporidium
Dear All,
I am looking into detecting giardia and cryptsporidium cysts and oocysts in
environmental samples using flow cytometry. However, due to safety
constraints, I need to inactivate the cysts and oocysts prior to running
them through the cytometer. Does anyone have related experience in this
area? What are the methods that are used to inactivate these parasites
whilst inflicting minimum changes in them?
Thank you.
Regards
Neo Sim Yee, Lezlie
Civil Engineering Laboratory
National University of Singapore
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