Hi Flowers, I would like to make chromosome sorting (from Drosophila) in our FACSVantage. The problem is that we don't have a 457 nm laser for Chromomycin A3 excitation (we have 488/UV dual laser) to achieve Hoechst/Chromomycin A3 dual staining . I have read a posted message in this list last year that Howard Shapiro replied and he commented that the Los Alamos group was working in bivariate histograms from 488 and UV lines staining with Hoechst and TOTO or YOYO (Cytometry 15:129-140, 1994). Is the resolution with Hoechst/TOTO or YOYO similar to Hoechst/Chromomycin A3? Could I really work with 488/UV laser for a good bivariate analysis and following sorting? Thanks in advance Merry Christmas and Happy Y2K to all ;-) Marco A. Fernández Head Cytometry Unit University Hospital Germans Trias i Pujol 08916 Badalona (Barcelona) Spain marcoa@ns.hugtip.scs.es http://personal.redestb.es/marcoafdez
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