Anyone out there try this product yet? How does it perform? Much tweaking? (yes, I sound like a broken record on another FACS bead assay....) :-) Cytokine Secretion Assays - for antigen-specific T cells The Cytokine Secretion Assays are a new labeling system for the enrichment and detection of human Cytokine secreting T-cells. An affinity matrix for the secreted product is generated by attaching an Cytokine specific Catch Reagent to the cell surface. Subsequently, the cells are incubated and the secreted Cytokine binds to the Catch Antibody. The caught cytokine, thus, represents an artificial surface marker which can subsequently be labeled using a PE-conjugated anti-Cytokine antibody (Detection Antibody). Magnetic labeling of the cells can now be achieved using Anti-PE MicroBeads. The Cytokine Secretion Assays can be used for the isolation of Cytokine secreting cells, in general. They can even be used to isolate antigen-specific, cytokine-producing T-cells after antigen specific stimulation. Enrichment of cytokine producing cells can easily be achieved on MACS columns type MS+/RS+ or LS+/VS+. -- Bunny Cotleur Cleveland Clinic Foundation Neurosciences NC30 216-444-1164 email: cotleua@ccf.org ****************************************************************** When you do something, you should burn yourself completely, like a good bonfire, leaving no trace of yourself. (Shunryu Suzuki)
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