Hi John, It's probably that visible part of the SIP on your problematic Calibur is longer than on the other one and it jams at the narrow bottom of cluster tubes. Fortunately, I think this is easily adjustable as I've noticed during the Bal seal replacement. Just unscrew the retainer at the top of the SIP and push the outer sleeve up as far as it will go and retighten the retainer. Before and after the adjustment, check the position of the SIP tip in the 12x75 tube by pushing the tube to the uppermost position. Hope this helps, Sasha On Wed, 01 Dec 1999 20:52:40 -0500 John Altman <altman@microbio.emory.edu> wrote: > > We have two FACS Caliburs with autoloaders, and most of the people in our > center love the autoloader. However, we have one problem on one but not the > other instrument. > > We do most of our staining in 96 well plates, and after washing the cells, > we use a multichannel pipettor to transfer the cell suspensions to 1 ml > cluster tubes. We then simply drop the cluster tubes inside a 12x75 FACS > tubes that are already arranged in the rack. On one of our Caliburs, we can > run the tubes this way with no problems. On the other, however, the cluster > tubes occasionally don't come down with the 12x75 tube when the lifter is > lowered, and then the cluster tube gets jammed in the next tube. We've tried > to gently bend the SIP to better align it, but this hasn't worked. > > Has anyone else faced this problem, and solved it? > > Thanks, > > John > > > *************************************************************************** > John D. Altman, Ph.D. > Emory University Vaccine Center at Yerkes > 954 Gatewood Road > Atlanta, GA 30329 > > Office: (404) 727-5981 > FAX: (404) 727-9005 > Lab: (404) 727-8914 > > email: altman@microbio.emory.edu > > *************************************************************************** > Tetramer Core Facility: > http://www.niaid.nih.gov/reposit/tetramer/index.html > *************************************************************************** > ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:19 EST