pH measurements

From: Snezna Rogelj (snezna@nmt.edu)
Date: Tue Dec 07 1999 - 15:01:38 EST

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Hello, Oh, You Wise (Wo)Men of the FlowLand:

Can a FlowCytometer be used to measure pH changes caused by an enzyme in a (water) solution?
I'd like to follow catalytic activity of carbonic anhydrase which induces a rapid (in seconds) drop in the water pH. Perhaps by monitoring beads conjugated to a pH-sensitive fluorescent dye? 

Your help is much appreciated,

Snezna

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