From: Barren, Phil [SMTP:BarrenP@MedImmune.com] Sent: Monday, December 06, 1999 8:46 AM To: 'Leary, James F.' Subject: RE: rare event DOes this mean that you don't believe the data on the 5 cells?/ Pb Phil, if you are asking if the cells were really there, yes they were.(not "probably") That was the distribution in the original sample, which was then put through the MACS,and then enriched to above 95% on the Vantage. I wasn't too concerned about the accuracy of the percentage given; as we were only interested in isolating them. The paper was published in Bone Marrow Transplant 1999 May;23(10):1019-1027 Authors;Kiel K, Cremer FW, et al. So back to rare cell sorting: we've recently acquired a high speed cell sorter(MoFlo); however I still prefer to use the MACS for enrichment of rare cells as at least this way hardly any cells get lost. With the Vantage the recovery of the rare cells was very low when compared to pre-enriching with the MACS; I don't know if this is so with high speed sorting, I think it would be interesting to know if anyone of you has experience here. regards Ann
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