Hello Everyone out in Flow-Land Back to Flow Cytometry 101... I've been having a debate with our grad student on the appropriate way of reporting results of 2-colour staining. He is staining activated T cells with a PE-conjugated activation marker and another FITC-conjugated monoclonal. He wants to make a statement on Activated vs. Non-activated cells. He prints up contour graphs with both colours and calls the UR quadrant his "FITC+ on activated cells" and the LR quadrant his "FITC+ on non-activated cells". I think he should be gating on activated cells (with FL2 histogram) and then produce an FL1 histogram to get the "FITC+ on activated cells". (Similar with non-activated cells, of course.) The numbers are substantially different depending on the method you use. Help please! Paula Lavery Transplant Immunology Lab. Royal Victoria Hospital McGill University Montreal (QC) Canada plaver@po-box.mcgill.ca "If we knew what we were doing it wouldn't be called research, would it?" -- Albert Einstein
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:18 EST