You may want to check all of your connectors. On occasion, we'll see the male/female connectors go bad, and that causes all kinds of headaches, from loss of vacuum on the Vantage, to backflush of waste on the Scans and Calibur. Hopefully you will find your problem there. Dax Arguello Huntsman Cancer Institute Flow Cyometry Core Facility Salt Lake City, UT (801) 581-8641 dax.arguello@hci.utah.edu -----Original Message----- From: ray hester To: cyto-inbox Sent: 11/29/99 8:35 AM Subject: vacuum on a Vantage Hi, We have a FACSVantage and have recently been having problems with drainage of the center stream, i.e., after thirty minutes or an hour the drainage has stopped, and we then have sheath fluid overflowing the center stream collector and fluid filling the basin below. Since we don't have house vacuum, we use a small Gast pump as the vacuum source and for awhile that worked well until we noticed water was getting into the pump. We then installed a trap in between the Vantage and the pump and for awhile that helped but the problem eventually re-surfaced. Next we filled the trap (a large, Erlenmeyer flask) with Drierite and that seemed to help for awhile but now nothing seems to work. Does anyone have a similar situation - Vantage and no house vacuum? If so, please let us know what your setup is. Perhaps the pump has been damaged beyond repair. Although it feels as if there is sufficent vacuum, I haven't actually checked to see how much vacuum there is and this is one of those small pumps without the gauges. Thanks for any ideas. Ray
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