Dear flowers, We are currently using a 4 colour panel with FITC, PE, RPE-Cy5 and APC on a FACS Calibur and have to compensate as much as 86% FL4-FL3 to get reasonable populations. This worked until a new antibody batch and combination differed too much in flourescense. Also we are not happy with this high compensation. Markers of interest are FITC and PE labeled, but we would like to use the FL3 and FL4 for more than just gating purposes. Looking in the archive there is only an unanswered post from 1995, claiming that it probably is impossible NOT to use PerCP but tandem conjugates instead. Any others using this setup? Any advices? Thanks in advance. With best regards, Katja ************************************************** Katja Adolf, PhD student Aarhus University Hospital Allergy and Lung Research Clinic Nørrebrogade 44, DK-8000 Aarhus C, Denmark Phone +45/ 8949 2106 Fax +45/ 8949 2110 email: kad@mailme.dk **************************************************
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