Intracellular cytokine flow

From: Jian G Zhang (Jian.G.Zhang@Unilever.com)
Date: Thu Nov 25 1999 - 04:17:04 EST


Dear All,

I wish to get some advice from someone who has been working on intracellular
cytokine flow cytometry using rat lymphocytes. I like to initially use
commercial reagents for washing, fixation and permeabilization steps. Since
there are so many available and they are mostly for human cells, can you
recommend any particular reagents for using in the rat? 

Many thanks for your help.

Dr Jian G Zhang
SEAC Toxicology
Unilever Research
Colworth House
Sharnbrook
Bedford,  MK44 1LQ
UK
Tel: +44 1234 222537
Fax: +44 1234 222122



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