the stuff is deadly for sorts, we checked on that. if you enquire with B-D they will also tell you the same thing. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Wed, 24 Nov 1999, Carl-Magnus Hogerkorp wrote: > > Dear Friends, > > For both sorting and analysis we use sheath fluid sold under the name "FACS > Flow" by BD (in Europe). This, I've been told, is basically PBS with an > azide component as preservative. Generally this works fine but when it comes > to single cell sorts we tend to believe that the small amount of FACS Flow > that falls into the 20µl Terasaki well has detrimental effects on the cell. > What are your experiences with this reagent? What are you using and from > what suppliers? Has the azide component any positive/negative effects on the > analyzed material or is it simply there as an antimicrobial agent? > > Thank you for your answers. > > Carl-Magnus Högerkorp > Stem Cell Laboratory > University Hospital of Lund > Sweden > >
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