Steve, I bought a diode pumped green laser (100mW) from a company called Casix, for approx. 5000 dollars.The dimension of the laser head is 75mm*44mm*155mm. expected lifetime 10,000hrs. When I need to use it (which isn't very often) I just set it up in front of either of my first or second lasers and use their steering prisms. The company's address in the States is: Casix 1933 South Myrtle Ave. Monrovia, CA91016 USA tel:(818) 305-1161 fax. (818)305-1163 e-mail: casixus@aol.com regards Ann Atzberger EMBL Heidelberg At 22:04 18.11.99 +0100, you wrote: > >Beware with Green HeNe Lasers since their power is very low (no more than 2 >mW). I had one on my ELITE, and Coulter France was unable to adjust it fine. >If you want to use FITC (488 nm Argon laser) and phycoerythrine (544 nm Green >HeNe), you absolutely need a Notch Filter (544 blocking) to avoid side >scatter light in PMT2 (the one for FITC). This filter is very expensive, and >reduce all fluorescences. Some people find that green HeNe work well, but >this not in France. Dr Egeberg in Norway is very happy with it. >Good luke > >Philippe Bourin > >"Steve G. Hilliard" wrote : > >> Hi folks, >> >> I need info on adding a green HeNe to my Elite Analyzer. Has anyone done >> this, on their own or through Beckman Coulter? Any info would be >> appreciated--I need to get them an estimated price for a proposal ASAP. >> I assume I'll need mounts and steering filters, but what about power? >> >> On a related note, has anyone gotten through to B-C Miami today? I've >> been calling (800) 327-6531, and I get the answering system, but >> never get an operator...Just when I wanted to buy something! ;-) >> >> Steve >> +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ >> Steve G. Hilliard (706) 542-9474 >> University of Georgia Cell Analysis Facility >> flowman@uga.edu http://floweb.cb.uga.edu/ > >
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