Sarah, A helpful point about intracellular staining is that in general all the fix/permeabilization procedures available, either commercial or home brew, will cause a change in the scatter properties of the cells. Slight adjustments in your settings on the controls I know you run will bring back your populations and this is perfectly acceptable. Have fun, Randy T. Fischer TherImmune Research Corporation 9700 Great Seneca Hwy Rockville, MD 20850 (240) 453-6256 RFischer@therimmune.com > ---------- > From: Sarah Lawson > Sent: Saturday, November 13, 1999 5:33 AM > To: Cytometry Mailing List > Subject: Re:Intraprep reagent & light scatter > > Dear All, > > I am relatively new to flow cytometry and have a query regarding > Coulter Immunotech Intraprep reagent. Has anyone got any experience > with this product? We are finding a quite marked change in scatter > properties following its use, although we can obtain the same scatter > pattern by increasing FS and SS total gain. Does this matter? > > Any comments would be gratefully received. > > Kind regards, > > Sarah Lawson > ( isaac.lawson@btinternet.com) > > >
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