Dear colleagues, > we are currently investigating the role of apoptosis in neutrophils from > BAL and sputum in patients with COPD. Initially, we perform a cell count > and viability using a haemocytometer and trypan blue. We then label with > Annexin V-FITC and propidium iodide (PI), and analyse on a Coulter flow > cytometer. To date, we have seen quite considerable discrepancies > between viability by trypan blue exclusion and that measured by PI > binding. We wondered if anyone has had any experience in this area or knowledge as to why this might be. To your knowledge, which test of viability do you think would more accurately reflect the true cell viability? > We would appreciate any help/comments greatly as regards this matter. > Thank you. Yours sincerely, > > > Martin Kelly ---------------------- Martin Kelly Department of Clinical Biochemistry, Institute of Clinical Science, Grosvenor Road, BELFAST. BT12 6BJ Tel No: (01232) 263267
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