Re: synchronizing T cells.

From: Andrew D. Wells, Ph.D. (adwells@mail.MED.UPENN.EDU)
Date: Wed Nov 17 1999 - 15:12:16 EST

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>Hi people!!!!
>
>Does anybody knows any protocol to sychronize T cells in order to measure
>the cell cycle time?


Mimosine (250 microM) will arrest T cells in G1.  There are certainly a
myriad of drugs that one could use, but I've found mimosine to have certain
advantages; cells can remain arrested for several days without appreciabe
loss in viability, and the arrest is reversible.

AW


Andrew D. Wells, Ph.D.
University of Pennsylvania
Department of Medicine
904 Stellar-Chance Laboratories
422 Curie Boulevard
Philadelphia, PA  19104
(215) 898-1951
(215) 573-2880 (FAX)
adwells@mail.med.upenn.edu

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