>Does anyone have any info on labeling transduced proteins in on the surface >of insect cells??? I have an investigator who wants to look at Sf9 cells >and find her protein. Has anyone used insect cells in flow??? > >Charles A. Kuszynski, Ph.D. >Assistant Professor/Director >University of Nebraska Medical Center >986495 Nebraska Medical Center >Cell Analysis Facility, WH 3024 >Omaha, NE 68198-6495 >402.559.6267 voice >402.559.4077 fax >ckuszyns@unmc.edu Hi Charles, I sort drosophila and mosquito cell lines. No problem as long as sample is suspended in optimal medium and sorted into optimal medium. Done at room temperature. Have sorted at normal speed with the Vantage and high speed at 40psi with the moFlo. Ann Atzberger Flow Cytometry Service Facility EMBL Heidelberg Germany.
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