Charles, I cultured sf9 for years, but the only flow I've done with them was a quick demonstration of DNA content using PI. That worked well. I would imagine it would work as well as any other system, perhaps better, since you don't need to trypsinize them, just shake or pipette gently. And if the protein is under the control of the baculovirus polyhedrin promoter you should get tons of signal. Good luck, Steve +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard (706) 542-9474 University of Georgia Cell Analysis Facility flowman@uga.edu URL: http://floweb.cb.uga.edu/ On Mon, 15 Nov 1999, ckuszyns@UNMC.EDU shared the following... > > > Does anyone have any info on labeling transduced proteins in on the surface > of insect cells??? I have an investigator who wants to look at Sf9 cells > and find her protein. Has anyone used insect cells in flow??? > > Charles A. Kuszynski, Ph.D. > Assistant Professor/Director > University of Nebraska Medical Center > 986495 Nebraska Medical Center > Cell Analysis Facility, WH 3024 > Omaha, NE 68198-6495 > 402.559.6267 voice > 402.559.4077 fax > ckuszyns@unmc.edu >
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