We have used this combination on the ACAS 570 for insitu hybridization techniques looking at Chromosome 8 and c-myc expression. We used the following filter combinations in the detector box. Dichroic mirror 575sp PE band pass 568 LP FITC Standard 530/30 ref: Perry, G.A., Kuszynski, C., Wang, Z., Sun, J. and Dicke-Evinger, M.J.; Simultaneous Detection and Quantitation of Chromosome Ploidy and Cytoplasmic mRNA Expression by Fluorescence in-situ Hybridization. Cytom., supp 6, 8C, 1993. Charles A. Kuszynski, Ph.D. Assistant Professor/Director University of Nebraska Medical Center 986495 Nebraska Medical Center Cell Analysis Facility, WH 3024 Omaha, NE 68198-6495 402.559.6267 voice 402.559.4077 fax ckuszyns@unmc.edu
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