We've had a FACSCalibur with a red-diode laser for over two years and it still works. And we use it very infrequently. David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center ---------------------- Forwarded by David McFarland/VUMC/Vanderbilt on 11/11/99 02:34 PM --------------------------- Alice.L.Givan@Dartmouth.EDU (Alice L. Givan) on 11/11/99 07:39:04 AM (Embedded image moved to file: pic23334.pcx)From:(Embedded image moved to file: pic05996.pcx)Alice.L.Givan@Dartmouth.EDU (Alice L. Givan) on 11/11/99 07:39 AM To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc: (bcc: David McFarland/VUMC/Vanderbilt) Subject: red diodes Hi Flowers, What are people's experiences with red diode lasers? I have been told that the manufacturers rate their lifetimes at 15,000 hours --- but that they seem to need replacing in cytometers after about 6 months. Is this true? Any ideas as to what is going on ? Thanks! Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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